Recently, researchers from the Stomatology Hospital of Xi’an Jiaotong University and the stomatology School of the Fourth Military Medical University jointly published a paper to explore the effect of phenytoin sodium (PHT) on the expression of calmodulin (CAM) in human periodontal ligament stem cells under the environment of endotoxin (LPS).
this study points out that PHT may promote the proliferation of periodontal ligament stem cells through the cam pathway The mixed stimulation effect of low concentration PHT and LPS of
is better than that of high concentration PHT and LPS.
was published in the Journal of practical dentistry, Vol. 28, 04, 2012.
The method of this study is to culture human periodontal ligament stem cells in vitro, and set up 6 experimental groups: blank control, LPS 5 mu; g / ml, pht5 mu; g / ml, pht20 mu; g / ml, pht5 mu; g / ml + LPS 5 mu; g / ml and pht20 mu; g / ml + LPS 5 mu; g / ml. after stimulation for 24 hours, 3 days and 5 days, RT The expression of cam in periodontal ligament stem cells was detected by PCR, immunofluorescence cytochemistry and confocal microscopy
The results of this study are as follows: (1) single stimulation of PHT, LPS and co stimulation of PHT and LPS can enhance the expression of cam in periodontal ligament stem cells.
② pht20 mu; g / ml has the strongest positive effect on the production of cam in periodontal ligament stem cells. The mixed stimulation of
③ pht5 mu; g / ml + lps5 mu; g / ml is stronger than pht20 mu; g / M + lps5 mu; g / m.