Recently, orthodontics researchers from the school of Stomatology, the Fourth Military Medical University published a paper to observe the effects of three-dimensional rotational simulated weightlessness on the morphology and function of human periodontal ligament fibroblasts (HP DLFS); h) The cytoskeleton structure and nucleus morphology of hPDLF cells changed after exposure to three-dimensional rotating culture conditions, but with the prolongation of stimulation time (72 & nbsp; H), the cells returned to normal and showed the trend of increasing cell viability and proliferation ability.
was published in the second issue of aerospace medicine and medical engineering in 2011.
HPDLF cells were cultured with TDC for 48, 72 and 96 hours respectively in the rotating group, while those in the control group were cultured with HE staining, cell counting, MTT activity detection, flow cytometry, Hoechst & nbsp; for
cells; 33258 nuclear staining and immunofluorescence analysis were used to compare the biological properties of the two groups.
Compared with the control group, the cell nuclear area of hPDLF cells in three-dimensional rotation culture was smaller (P & lt; 0.01), the microfilament structure was slightly fuzzy, but the cell proliferation and apoptosis were not significantly changed; after 72 & nbsp; H rotation, the cell viability was significantly enhanced (P & lt; 0.01), but the cell morphology and apoptosis were not significantly changed; 96 & nbsp; After h, the proliferation ability of hPDLF cells was significantly enhanced (P & lt; 0.01), the cell viability was significantly increased (P & lt; 0.01), the proportion of S-phase cycle was significantly increased (P & lt; 0.05), and there was no significant difference in cell morphology and apoptosis between the control group and the control group
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